Storage method of peptides

Release time:


Peptide based health foods only need to be placed in a cool and dry place.

For peptide reagents and peptide drugs, they need to be stored in a dryer. Before exposing them to air, freeze-dried peptides can be placed at room temperature. This will reduce the impact of humidity, and when freeze-drying is not possible, the method is to store it in a small working sample size. For peptide reagents containing Cys, Met, or TrP, deoxygenation buffer is essential for their dissolution, as these peptide reagents are easily oxidized by air. Before sealing the bottle, slowly flowing nitrogen or argon gas through the peptide reagent can also reduce the oxidation effect. Peptide reagents containing Gln or Asn are also prone to degradation, and all these peptides have a limited lifespan compared to those without these problematic glycosides.

Peptides have wide solubility. The main problem with insoluble peptides is the formation of secondary structures. Except for peptides, this can occur more significantly in peptides with multiple hydrophobic residues. Salt promotes the formation of secondary structures. We suggest dissolving the peptide in sterile distilled water or deionized solution first. If it is necessary to increase the dissolution rate, ultrasonic treatment can be used. There is still a problem with dissolution, adding a small amount of dilute acetic acid (10%) or ammonia solution will facilitate dissolution.

Peptide reagents should be stored for a long time, freeze-dried, and cold dried powder can be stored at -20 ℃ or lower for several years with little or no degradation. The peptides in the solution are far from stable. Peptides are easily degraded by bacteria and should be dissolved in sterile purified water.

Peptide solutions containing Met, Cys, or Tyr residues have limited lifespan due to oxidation. It should be dissolved in anaerobic solvents. To prevent damage from repeated freeze-thaw cycles, it is recommended to dissolve excess peptides in fecal experiments and store the remaining peptides in solid form.